Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease

doi: 10.1152/ajpheart.00362.2013

Figure Lengend Snippet: Measurements with laser Doppler perfusion imaging (LDPI) and the OxyLite tissue oxygen tension sensor confirm time course of recovery in ischemic limb. A: representative LDPI image of ischemic (bottom) and control (top) footpads immediately postsurgery. The white “x” indicates the approximate location of the distal ligation site. B: LDPI ratios demonstrate full recovery of perfusion in the ischemic footpad relative to the control footpad by day 21 (means ± SE with n = 15). C: absolute Po2 values from control and ischemic footpads measured with the OxyLite system (means ± SE with n = 5). D: individual animal ratios (ischemic Po2 normalized to mean control Po2 for each animal) plotted with lines representing mean ratio ± SE at each time point. *One-sided P < 0.05 and **P < 0.01 as determined by a Wilcoxon Signed-Rank test (B–D) followed by a Bonferroni correction for multiple comparisons (B, D) with respect to the corresponding control measurement (C) or the preceding time point (B, D).

Article Snippet: Tissue oxygen tension (P o 2 ) was measured in the footpads of five mice under isoflurane anesthesia and maintained at normal body temperature at 0, 3, 7, 14, and 20 days postsurgery using the commercially available fiber-optic OxyLite (Oxford Optronix) tissue oxygenation sensor.

Techniques: Imaging, Control, Ligation

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease

doi: 10.1152/ajpheart.00362.2013

Figure Lengend Snippet: OxyLite measurements are variable at normal tissue Po2 levels. OxyLite tissue oxygenation sensor measurements are more precise at low tissue Po2 and show high spatial variability at normal Po2 levels. Tissue Po2 data are shown from each animal for 2 locations on each footpad at day 0 (A), day 3 (B), day 7 (C), and day 20 (D). Data presented as means ± SD of 7 readings in each location collected over 30 s.

Article Snippet: Tissue oxygen tension (P o 2 ) was measured in the footpads of five mice under isoflurane anesthesia and maintained at normal body temperature at 0, 3, 7, 14, and 20 days postsurgery using the commercially available fiber-optic OxyLite (Oxford Optronix) tissue oxygenation sensor.

Techniques:

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease

doi: 10.1152/ajpheart.00362.2013

Figure Lengend Snippet: Vascular endpoints in the hind limb ischemia (HLI) model are positively correlated and exhibit differences in time rate of recovery due to measurement of distinct physiological processes. Correlations between hyperspectral imaging measurement of hemoglobin oxygen saturation (HbSat) and LDPI perfusion (A) and OxyLite Po2 ratios (B) and between the Doppler OCT vascular index and LDPI perfusion (C) and HbSat ratios (D) are shown. In A, C, and D, the data include measurements from all mice at all time points. In B, mean values for each time point are compared since the invasiveness of the OxyLite sensor necessitated the use of a separate cohort of mice. The Pearson correlation coefficients for all 4 comparisons are significant (P < 0.01).

Article Snippet: Tissue oxygen tension (P o 2 ) was measured in the footpads of five mice under isoflurane anesthesia and maintained at normal body temperature at 0, 3, 7, 14, and 20 days postsurgery using the commercially available fiber-optic OxyLite (Oxford Optronix) tissue oxygenation sensor.

Techniques: Imaging

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease

doi: 10.1152/ajpheart.00362.2013

Figure Lengend Snippet: Interanimal variability is smallest in hyperspectral imaging and largest in OxyLite Po2 measurements. Hyperspectral imaging of hemoglobin oxygen saturation shows more consistent trends across all animals measured than both LDPI perfusion and OxyLite Po2 measurements in this model. A: hemoglobin saturation ratio data for n = 15 animals over 21 days shows a consistent increasing trend. B: LDPI perfusion ratio data for n = 15 animals over 21 days shows an increasing trend with high interanimal variation. C: OxyLite Po2 ratio data for n = 5 animals over 20 days is highly variable and lacks a consistent increasing or decreasing trend across all animals. D: Doppler OCT vascular index ratios for n = 8 animals show interanimal variability in the recovery of blood flow through vessels in the distal femoral artery region (area 1) and a slower temporal response, indicating that the greater imaging depth of OCT provides insight into residual flow deficits not detectable by the superficial measurements obtained with hyperspectral imaging, LDPI, and OxyLite Po2. This may represent a useful metric for analysis of the later stages of recovery. The average standard error over the time course is indicated by SEMavg for each technique.

Article Snippet: Tissue oxygen tension (P o 2 ) was measured in the footpads of five mice under isoflurane anesthesia and maintained at normal body temperature at 0, 3, 7, 14, and 20 days postsurgery using the commercially available fiber-optic OxyLite (Oxford Optronix) tissue oxygenation sensor.

Techniques: Imaging